HYDROLYSIS OF FISH OIL BY HYPERACTIVATED Rhizomucor miehei IMMOBILIZED BY MULTIPOINT ANION EXCHANGE
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Immobilization of Rhizomucor miehei Lipase on High Density Polyethylene
Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...
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Upgrading of farmed salmon oil obtained from by-products was carried out by lipase-catalyzed hydrolysis to increase omega 3 polyunsaturated fatty acids (PUFA) content. The lipases tested were from Penicillium camembertii (PC), Rhizomucor javanicus (RJ), Rhizopus niveus (RN), Rhizopus delemar (RD), Burkholderia cepacia (BC), Rhizopus oryzae (RO), Candida rugosa (CR) and Rhizomucor miehei (RM). T...
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A new assay was designed to measure the release of omega-3 acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid ( DHA)) from the hydrolysis of sardine oil by lipases immobilized inside porous supports. A biphasic system was used containing the fish oil dissolved in the organic phase and the immobilized lipase suspended in the aqueous phase. The assay was optimized by using a very active ...
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* corresponding author. e-mail: [email protected] Phenethyl octanoate (PEO) is a natural flavour compound found in rum, spirits and wine. Six commercial and non-commercial lipase preparations were screened for 2-phenethyl octanoate synthesis activity, the most active enzymes were Lipozyme® RM IM and Palatase® 20000 L (immobilized and soluble Rhizomucor miehei lipases). Three substrates a...
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